Research Project Database
Code: EMIDA3
Title: LA-MRSA - Methicillin-resistant Staphylococcus aureus lineages in primary productions: multi-host pathogen, spill-over and spill-back between animals and humans?
Country: Denmark
Funding Organisation: Veterinary and Agrochemical Research Centre (CODA-CERVA)
Federal Ministry of Education and Research (BMBF, Germany)
Italian Ministry of Health Department for Veterinary Public Health, Nutrition and Food Safety
Animal Group:  
Pathogen: Methicillin Resistant Staphylococcus aureus (MRSA)
Category: Epidemiology, Risk and Decision Support > Descriptive epidemiology
Epidemiology, Risk and Decision Support > Generic/Fundamental epidemiology and modelling
Infection, immunity and biotechnology > Diagnostic test development, including microarray
Infection, immunity and biotechnology > Fundamental/basic and Applied studies, including molecular, cellular, pathogen population dynamics and ecology > Bacteriology
Infection, immunity and biotechnology > Fundamental/basic and Applied studies, including molecular, cellular, pathogen population dynamics and ecology > Molecular epidemiology
Research Organisation: Veterinary and Agrochemical Research Centre (CODA-CERVA)
Technical University of Denmark
Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna
BfR - Federal Institute for Risk Assessment
Istituto Zooprofilattico Sperimentale del Piemonte - Liguria e Valle d'Aosta
Istituto Zooprofilattico Sperimentale delle Regioni Lazio e Toscana
CONGEN Biotechnologie GmbH
Centro de Vigilancia Sanitaria Veterinaria (VISAVET)
Number of Research Staff (FTE):  
Principal Investigator (PI): Antonio Battisti, Istituto Zooprofilattico Sperimentale delle Regioni Lazio e Toscana
Cost (Euros): 1709100
End Date (dd/mm/yyyy): 31-12-2013
Duration (months): 24
Project objectives and deliverables with estimated delivery dates for each deliverable (if possible): Methicillin-resistant Staphylococcus aureus (MRSA) is a leading cause of hospital-associated infections and has emerged recently as a significant pathogen in EU. MRSA clones recently emerged in livestock now referred to as Livestock-Associated MRSA (LA-MRSA). Different MRSA clones have found an ecological niche in pig farming and other livestock in many European countries. Preliminary studies show a remarkable difference in distribution of different LA-MRSA types between countries, suggesting regional influences on the epidemiology. A two-year ongoing study in 4 different European countries will provide useful information about heterogeneity and epidemiology of MRSA in these MSs.
The Objectives of the Project are the following:
1 assessing the diffusion and the relationships of LA-MRSA clones in primary productions in EU, as well as the diffusion of MRSA lineages other than those known to be associated with farm animals (i. e. Clonal Complex 398), with particular attention to lineages of human origin circulating in farm animals; 2 assessing the presence of different multi-resistance patterns and its distribution among different MRSA clones and lineages; 3 investigating the possible association between the use of some antimicrobials (including metals) and the presence and prevalence of MRSA in animal farming in view of possible intervention; 4 providing genotypic characterisation of MRSA clones obtained by means of population studies in farm animals (i. e. spa-types, Sequence Types, and the presence virulence and resistance determinants) also by the use of innovative and multiple molecular diagnostic assays, for molecular epidemiology purposes; 5 Development and validation of innovative real-time PCR based screening methods for the detection of MRSA clones from livestock and for selected virulence and resistance determinants. , which could consequently be used as sensitive tools to obtain comparable data on the prevalence and characteristics of MRSA clones. 6 providing colonisation experiments in pigs for selected non-CC398 lineages, known to circulate in pig farms in some MSs; 7 exploring the possible patterns of diffusion of MRSA colonization within holdings; 8 investigating possible risk factors for the positive status of holdings from LA-MRSA lineages and human-associated MRSA lineages circulating among farm animals by epidemiological studies; 9 comparing frequencies and genotype profile of isolates detected in animals and those of the same genotype profile detected in humans, through passive surveillance or early warning on the occurrence of infections or contamination of humans by selected group of lineages, either of animal or human origin; The results delivered by the project will be important in understanding the epidemiology of MRSA clones circulating in farm animals. Additionally, it will provide information on the occurrence of the spill-over spill-back phenomenon between farm animals and humans, for risk assessment purposes.
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