Research Project Database
Code: 1.18.04
1: 1.18.04
Title: Evaluation of the cut-off point of the interferon-y assay (IFN-γ) for eradication of bovine tuberculosis.
2: Evaluation of the cut-off point of the interferon-y assay (IFN-γ) for eradication of bovine tuberculosis.
Country: Switzerland
3: Switzerland
Funding Organisation: Federal Food Safety and Veterinary Office FSVO
4: Federal Food Safety and Veterinary Office FSVO
Animal Group: Cattle
5: Cattle
Pathogen:  
6:  
Disease:  
7:  
Category:  
8:  
9:  
Research Organisation: Institute of Veterinary Bacteriology
University of Zurich (UZH)
10: Institute of Veterinary Bacteriology
University of Zurich (UZH)
Number of Research Staff (FTE):  
11:  
Principal Investigator (PI):  
12:  
Cost (Euros): 116935
13: 116935
End Date (dd/mm/yyyy): 30-06-2019
14: 1561849200
Duration (months): 18
15: 18
Link:  
16:  
Project objectives and deliverables with estimated delivery dates for each deliverable (if possible): The IFN-y assay has been used worldwide as an ancillary test to the intradermal tuberculin test for diagnosis of bovine tuberculosis (bTB). The use of the IFN-γ assay has the advantage of detecting animals recently infected or not detected by the intradermal tuberculin test. The assay is based on the ELISA technology, detecting and quantifying the release of the IFN-γ cytokine when heparinised whole blood is incubated with bovine and avian PPD tuberculin within 8–24 hours post collection. According to the Swiss technical instruction for bTB, the IFN-γ assay is applied in cases where the intradermal tuberculin skin test leads to inconclusive results. The aim of this assay is to verify inconclusive results; therefore, it should have a high sensitivity. The use of more restrictive thresholds can increase the sensitivity of the assay but may negatively influence its specificity. In different countries, several cut-off points are currently being used. The threshold should be evaluated in each country based on the local epidemiological conditions.
17: The IFN-y assay has been used worldwide as an ancillary test to the intradermal tuberculin test for diagnosis of bovine tuberculosis (bTB). The use of the IFN-γ assay has the advantage of detecting animals recently infected or not detected by the intradermal tuberculin test. The assay is based on the ELISA technology, detecting and quantifying the release of the IFN-γ cytokine when heparinised whole blood is incubated with bovine and avian PPD tuberculin within 8–24 hours post collection. According to the Swiss technical instruction for bTB, the IFN-γ assay is applied in cases where the intradermal tuberculin skin test leads to inconclusive results. The aim of this assay is to verify inconclusive results; therefore, it should have a high sensitivity. The use of more restrictive thresholds can increase the sensitivity of the assay but may negatively influence its specificity. In different countries, several cut-off points are currently being used. The threshold should be evaluated in each country based on the local epidemiological conditions.
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