Research Project Database
Code: 127916
1: 127916
Title: Identification and isolation of economically important fish viruses and development of new generation prototype vaccines
2: Identification and isolation of economically important fish viruses and development of new generation prototype vaccines
Country: Hungary
3: Hungary
Funding Organisation: National Research, Development and Innovation Office
4: National Research, Development and Innovation Office
Animal Group: Fish
5: Fish
Pathogen:  
6:  
Disease:  
7:  
Category: Disease pathogenesis, including pathogen biology, evolution and resistance
8: Disease pathogenesis, including pathogen biology, evolution and resistance
9: 79
Research Organisation: Institute for Veterinary Medical Research (Centre for Agricultural Research)
10: Institute for Veterinary Medical Research (Centre for Agricultural Research)
Number of Research Staff (FTE):  
11:  
Principal Investigator (PI): Andor Doszpoly
12: Andor Doszpoly
Cost (Euros): 95333
13: 95333
End Date (dd/mm/yyyy): 26-10-2019
14: 1572117720
Duration (months): 48
15: 48
Link: http://aoti.agrar.mta.hu/en/projects_NKFI_K_127916
16: http://aoti.agrar.mta.hu/en/projects_NKFI_K_127916
Project objectives and deliverables with estimated delivery dates for each deliverable (if possible): Apart from the diagnosis of the OIE listed aquatic viruses, there is no research group specialized in fish virology in Hungary. The principal investigator, during his PD OTKA grant, has carried out research on the genetic analysis of several fish viruses. These results assisted to refine the taxonomy of fish viruses, and contributed to increase the knowledge on the diversity of fish viruses in Hungary. Some promising DNA vaccine candidates were also studied for sturgeon herpesvirus. We intend to continue these studies within the framework of this grant. This time, we would focus on the isolation of fish viruses occurring in Hungarian fish farms, which will provide the basis for the better understanding of the biology of these viruses and for selecting the best defense methods against them. For this purpose we need to establish novel permanent cell lines from domestic intensively reared fish species. Primary cells will be immortalized by viral gene transfection. After successful isolation, the novel virus will be characterized fully. The alloherpesviruses (koi herpesvirus, KHV) are important veterinary pathogens causing severe economic losses worldwide. These viruses are able to enter latency, during this period it is not possible to detect them by direct methods, so it would be highly desirable to develop a sensitive indirect virus detection method (ELISA) for screening the asymptomatic fish stock. New generation DNA vaccine prototypes will be designed and tested in animal experiments for economically important fish viruses (KHV, carp edema virus, CEV). For these latter purposes the first isolation and/or genetic analysis of Hungarian variants of these viruses are needed.
17: Apart from the diagnosis of the OIE listed aquatic viruses, there is no research group specialized in fish virology in Hungary. The principal investigator, during his PD OTKA grant, has carried out research on the genetic analysis of several fish viruses. These results assisted to refine the taxonomy of fish viruses, and contributed to increase the knowledge on the diversity of fish viruses in Hungary. Some promising DNA vaccine candidates were also studied for sturgeon herpesvirus. We intend to continue these studies within the framework of this grant. This time, we would focus on the isolation of fish viruses occurring in Hungarian fish farms, which will provide the basis for the better understanding of the biology of these viruses and for selecting the best defense methods against them. For this purpose we need to establish novel permanent cell lines from domestic intensively reared fish species. Primary cells will be immortalized by viral gene transfection. After successful isolation, the novel virus will be characterized fully. The alloherpesviruses (koi herpesvirus, KHV) are important veterinary pathogens causing severe economic losses worldwide. These viruses are able to enter latency, during this period it is not possible to detect them by direct methods, so it would be highly desirable to develop a sensitive indirect virus detection method (ELISA) for screening the asymptomatic fish stock. New generation DNA vaccine prototypes will be designed and tested in animal experiments for economically important fish viruses (KHV, carp edema virus, CEV). For these latter purposes the first isolation and/or genetic analysis of Hungarian variants of these viruses are needed.
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